Silver nanoparticles inhibit proliferation and migration of prostate cancer cells: an experimental report
WCRJ 2022;
9: e2242
DOI: 10.32113/wcrj_20223_2242
Topic: Genitourinary cancer
Category: Original article
Abstract
Objective: Prostate carcinoma is a serious public health in men with increasing incidence and mortality rates. The present study aimed to investigate cytotoxic activities of silver nanoparticles (AgNPs) against PC-3 human prostate cancer cell line in vitro.
Materials and Methods: The antiproliferative effect of AgNPs on PC-3 cells was assessed using various concentrations of AgNPs (5-80 µg/mL) by MTT assay. We have also investigated the effect of AgNPs on the migration property of cancers cells using a wound-healing assay. Then, obtained results were analyzed using ANOVA and Student’s t-test.
Results: The AgNPs diminished significantly the viability of PC-3 cells in a dose and time-dependent manner. According to the dose-dependent viability curve, the IC50 (50% inhibiting concentration) at 48 h was calculated to be 65.7 µg/mL. Migration assay also confirmed the antimetastatic potential of the AgNPs against PC-3 cells. After 24 h, the migration rate was 2.5 fold lower for AgNPs-treated cells compared to control cells.
Conclusions: Our findings support the use of AgNPs as an appropriate option for future therapeutic application in prostate cancer therapy.
Materials and Methods: The antiproliferative effect of AgNPs on PC-3 cells was assessed using various concentrations of AgNPs (5-80 µg/mL) by MTT assay. We have also investigated the effect of AgNPs on the migration property of cancers cells using a wound-healing assay. Then, obtained results were analyzed using ANOVA and Student’s t-test.
Results: The AgNPs diminished significantly the viability of PC-3 cells in a dose and time-dependent manner. According to the dose-dependent viability curve, the IC50 (50% inhibiting concentration) at 48 h was calculated to be 65.7 µg/mL. Migration assay also confirmed the antimetastatic potential of the AgNPs against PC-3 cells. After 24 h, the migration rate was 2.5 fold lower for AgNPs-treated cells compared to control cells.
Conclusions: Our findings support the use of AgNPs as an appropriate option for future therapeutic application in prostate cancer therapy.
To cite this article
Silver nanoparticles inhibit proliferation and migration of prostate cancer cells: an experimental report
WCRJ 2022;
9: e2242
DOI: 10.32113/wcrj_20223_2242
Publication History
Submission date: 04 Oct 2021
Revised on: 11 Nov 2021
Accepted on: 16 Feb 2022
Published online: 17 Mar 2022
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