Loop-mediated isothermal amplification (LAMP) and its variants as simple and cost effective for genotyping method

WCRJ 2018; 5 (3): e1116

  Topic: Pharmacogenomics     Category:

Abstract

Since the time of its introduction, the LAMP method has been usually used for the detection of infections in which just the amplification of the pathogen genome is required. The LAMP method is an Isothermal technique that amplifies the DNA segment of interest. In this review, different studies that used LAMP method as a technique for genotyping and SNPs detection were evaluated. These studies combined the LAMP method with various technologies in order to make it suitable for genotyping. Since the LAMP method is a sharp, fast and robust technique, it is suitable for use in routine diagnostics. It seems that LAMP method could be a reliable and cost-effective method for genotyping and further studies are recommended to use this approach for genotyping. In parallel with this features, new high-throughput platforms are spreading in the routine of clinical diagnostics, encouraged by the progressive lowering of their costs, the strong cost-effectiveness and their high sensitivity/specificity. Despite this technological advance, an important issue is represented by the validation and quality check of these tests, since they have extremely high standards of sensitivity and specificity. With appropriate controls, these assays can represent a powerful tool in the hands of laboratorists for the continuous improvement of standard Point Of Care Testing (POCT).

To cite this article

Loop-mediated isothermal amplification (LAMP) and its variants as simple and cost effective for genotyping method

WCRJ 2018; 5 (3): e1116

Publication History

Submission date: 27 Aug 2018

Revised on: 05 Sep 2018

Accepted on: 12 Sep 2018

Published online: 20 Sep 2018