MicroRNA detection in tumor tissue by in situ hybridization

WCRJ 2014; 1 (4): e407

  Topic: Molecular diagnosis     Category:

Abstract

In this paper the Authors describe the main functions of microRNAs (miRNAs) along with well acknowledged methods of their detection in biological materials. They include serum, plasma, urine, normal human samples and neoplastic tissues. The Authors also discusses about the relevance of bright field in situ hybridization (ISH) methods for miRNA detection in revealing the cells of origin of specific miRNAs. In particular, the paper highlights the automated ISH protocols for miRNA detection which have recently been proposed. These techniques will enable investigators to further explore the biological role of miRNAs.

To cite this article

MicroRNA detection in tumor tissue by in situ hybridization

WCRJ 2014; 1 (4): e407

Publication History

Published online: 21 Dec 2014